In vitro and in vivo inhibitory effects of some fungicides on catalase produced and purified from white-rot fungus Phanerochaete chrysosporium.
Identifieur interne : 000313 ( Main/Exploration ); précédent : 000312; suivant : 000314In vitro and in vivo inhibitory effects of some fungicides on catalase produced and purified from white-rot fungus Phanerochaete chrysosporium.
Auteurs : Berna Kavakç O Lu [Turquie] ; Leman TarhanSource :
- Artificial cells, nanomedicine, and biotechnology [ 2169-141X ] ; 2014.
Descripteurs français
- KwdFr :
- Antibactériens (pharmacologie), Antienzymes (pharmacologie), Catalase (antagonistes et inhibiteurs), Catalase (biosynthèse), Catalase (composition chimique), Catalase (isolement et purification), Cinétique (MeSH), Concentration en ions d'hydrogène (MeSH), Fongicides industriels (pharmacologie), Milieux de culture (composition chimique), Phanerochaete (croissance et développement), Phanerochaete (enzymologie), Phanerochaete (métabolisme), Stabilité enzymatique (MeSH), Techniques de culture (MeSH), Température (MeSH).
- MESH :
- antagonistes et inhibiteurs : Catalase.
- biosynthèse : Catalase.
- composition chimique : Catalase, Milieux de culture.
- croissance et développement : Phanerochaete.
- enzymologie : Phanerochaete.
- isolement et purification : Catalase.
- métabolisme : Phanerochaete.
- pharmacologie : Antibactériens, Antienzymes, Fongicides industriels.
- Cinétique, Concentration en ions d'hydrogène, Stabilité enzymatique, Techniques de culture, Température.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (pharmacology), Catalase (antagonists & inhibitors), Catalase (biosynthesis), Catalase (chemistry), Catalase (isolation & purification), Culture Media (chemistry), Culture Techniques (MeSH), Enzyme Inhibitors (pharmacology), Enzyme Stability (MeSH), Fungicides, Industrial (pharmacology), Hydrogen-Ion Concentration (MeSH), Kinetics (MeSH), Phanerochaete (enzymology), Phanerochaete (growth & development), Phanerochaete (metabolism), Temperature (MeSH).
- MESH :
- chemical , antagonists & inhibitors : Catalase.
- chemical , biosynthesis : Catalase.
- chemical , chemistry : Catalase, Culture Media.
- chemical , isolation & purification : Catalase.
- chemical , pharmacology : Anti-Bacterial Agents, Enzyme Inhibitors, Fungicides, Industrial.
- enzymology : Phanerochaete.
- growth & development : Phanerochaete.
- metabolism : Phanerochaete.
- Culture Techniques, Enzyme Stability, Hydrogen-Ion Concentration, Kinetics, Temperature.
Abstract
In this study, in vitro and in vivo effects of some commonly used fungicides, antibiotics, and various chemicals on isolated and purified catalase from Phanerochaete chrysosporium were investigated. The catalase was purified 129.10-fold by using 60% ammonium sulfate and 60% ethanol precipitations, DEAE-cellulose anion exchange and Sephacryl-S-200 gel filtration chromatographies from P. chrysosporium growth in carbon- and nitrogen-limited medium for 12 days. The molecular weight of native purified catalase from P. chrysosporium was found to be 290 ± 10 kDa, and sodium dodecyl sulfate (SDS)-PAGE results indicated that enzyme consisted of four apparently identical subunits, with a molecular weight of 72.5 ± 2.5 kDa. Kinetic characterization studies showed that optimum pH and temperature, Km and Vmax values of the purified catalase which were stable in basic region and at comparatively high temperatures were 7.5, 30°C, 289.86 mM, and 250,000 U/mg, respectively. The activity of purified catalase from P. chrysosporium was significantly inhibited by dithiothreitol (DTT), 2-mercaptoethanol, iodoacetamide, EDTA, and sodium dodecyl sulfate (SDS). It was found that while antibiotics had no inhibitory effects, 45 ppm benomyl, 144 ppm captan, and 47.5 ppm chlorothalonil caused 14.52, 10.82, and 38.86% inhibition of purified catalase, respectively. The inhibition types of these three fungicides were found to be non-competitive inhibition with the Ki values of 1.158, 0.638, and 0.145 mM and IC50 values of 0.573, 0.158, 0.010 mM, respectively. The results of in vivo experiments also showed that benomyl, captan and chlorothalonil caused 15.25, 1.96, and 36.70% activity decreases after 24-h treatments compared to that of the control.
DOI: 10.3109/21691401.2013.821412
PubMed: 24079700
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vitro and in vivo inhibitory effects of some fungicides on catalase produced and purified from white-rot fungus Phanerochaete chrysosporium.</title><author><name sortKey="Kavakc O Lu, Berna" sort="Kavakc O Lu, Berna" uniqKey="Kavakc O Lu B" first="Berna" last="Kavakç O Lu">Berna Kavakç O Lu</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Science, Department of Chemistry, University of Dokuz Eylul , Izmir , Turkey.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>Faculty of Science, Department of Chemistry, University of Dokuz Eylul , Izmir </wicri:regionArea><wicri:noRegion>Izmir </wicri:noRegion></affiliation></author><author><name sortKey="Tarhan, Leman" sort="Tarhan, Leman" uniqKey="Tarhan L" first="Leman" last="Tarhan">Leman Tarhan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24079700</idno><idno type="pmid">24079700</idno><idno type="doi">10.3109/21691401.2013.821412</idno><idno type="wicri:Area/Main/Corpus">000345</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000345</idno><idno type="wicri:Area/Main/Curation">000345</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000345</idno><idno type="wicri:Area/Main/Exploration">000345</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In vitro and in vivo inhibitory effects of some fungicides on catalase produced and purified from white-rot fungus Phanerochaete chrysosporium.</title><author><name sortKey="Kavakc O Lu, Berna" sort="Kavakc O Lu, Berna" uniqKey="Kavakc O Lu B" first="Berna" last="Kavakç O Lu">Berna Kavakç O Lu</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Science, Department of Chemistry, University of Dokuz Eylul , Izmir , Turkey.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>Faculty of Science, Department of Chemistry, University of Dokuz Eylul , Izmir </wicri:regionArea><wicri:noRegion>Izmir </wicri:noRegion></affiliation></author><author><name sortKey="Tarhan, Leman" sort="Tarhan, Leman" uniqKey="Tarhan L" first="Leman" last="Tarhan">Leman Tarhan</name></author></analytic><series><title level="j">Artificial cells, nanomedicine, and biotechnology</title><idno type="eISSN">2169-141X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (pharmacology)</term><term>Catalase (antagonists & inhibitors)</term><term>Catalase (biosynthesis)</term><term>Catalase (chemistry)</term><term>Catalase (isolation & purification)</term><term>Culture Media (chemistry)</term><term>Culture Techniques (MeSH)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Enzyme Stability (MeSH)</term><term>Fungicides, Industrial (pharmacology)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Kinetics (MeSH)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (growth & development)</term><term>Phanerochaete (metabolism)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antibactériens (pharmacologie)</term><term>Antienzymes (pharmacologie)</term><term>Catalase (antagonistes et inhibiteurs)</term><term>Catalase (biosynthèse)</term><term>Catalase (composition chimique)</term><term>Catalase (isolement et purification)</term><term>Cinétique (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Fongicides industriels (pharmacologie)</term><term>Milieux de culture (composition chimique)</term><term>Phanerochaete (croissance et développement)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (métabolisme)</term><term>Stabilité enzymatique (MeSH)</term><term>Techniques de culture (MeSH)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Catalase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Catalase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Catalase</term><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Catalase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Enzyme Inhibitors</term><term>Fungicides, Industrial</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Catalase</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Catalase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Catalase</term><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Catalase</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Antienzymes</term><term>Fongicides industriels</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Culture Techniques</term><term>Enzyme Stability</term><term>Hydrogen-Ion Concentration</term><term>Kinetics</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Concentration en ions d'hydrogène</term><term>Stabilité enzymatique</term><term>Techniques de culture</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, in vitro and in vivo effects of some commonly used fungicides, antibiotics, and various chemicals on isolated and purified catalase from Phanerochaete chrysosporium were investigated. The catalase was purified 129.10-fold by using 60% ammonium sulfate and 60% ethanol precipitations, DEAE-cellulose anion exchange and Sephacryl-S-200 gel filtration chromatographies from P. chrysosporium growth in carbon- and nitrogen-limited medium for 12 days. The molecular weight of native purified catalase from P. chrysosporium was found to be 290 ± 10 kDa, and sodium dodecyl sulfate (SDS)-PAGE results indicated that enzyme consisted of four apparently identical subunits, with a molecular weight of 72.5 ± 2.5 kDa. Kinetic characterization studies showed that optimum pH and temperature, Km and Vmax values of the purified catalase which were stable in basic region and at comparatively high temperatures were 7.5, 30°C, 289.86 mM, and 250,000 U/mg, respectively. The activity of purified catalase from P. chrysosporium was significantly inhibited by dithiothreitol (DTT), 2-mercaptoethanol, iodoacetamide, EDTA, and sodium dodecyl sulfate (SDS). It was found that while antibiotics had no inhibitory effects, 45 ppm benomyl, 144 ppm captan, and 47.5 ppm chlorothalonil caused 14.52, 10.82, and 38.86% inhibition of purified catalase, respectively. The inhibition types of these three fungicides were found to be non-competitive inhibition with the Ki values of 1.158, 0.638, and 0.145 mM and IC50 values of 0.573, 0.158, 0.010 mM, respectively. The results of in vivo experiments also showed that benomyl, captan and chlorothalonil caused 15.25, 1.96, and 36.70% activity decreases after 24-h treatments compared to that of the control.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24079700</PMID><DateCompleted><Year>2015</Year><Month>05</Month><Day>18</Day></DateCompleted><DateRevised><Year>2014</Year><Month>09</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2169-141X</ISSN><JournalIssue CitedMedium="Internet"><Volume>42</Volume><Issue>5</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Artificial cells, nanomedicine, and biotechnology</Title><ISOAbbreviation>Artif Cells Nanomed Biotechnol</ISOAbbreviation></Journal><ArticleTitle>In vitro and in vivo inhibitory effects of some fungicides on catalase produced and purified from white-rot fungus Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>356-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3109/21691401.2013.821412</ELocationID><Abstract><AbstractText>In this study, in vitro and in vivo effects of some commonly used fungicides, antibiotics, and various chemicals on isolated and purified catalase from Phanerochaete chrysosporium were investigated. The catalase was purified 129.10-fold by using 60% ammonium sulfate and 60% ethanol precipitations, DEAE-cellulose anion exchange and Sephacryl-S-200 gel filtration chromatographies from P. chrysosporium growth in carbon- and nitrogen-limited medium for 12 days. The molecular weight of native purified catalase from P. chrysosporium was found to be 290 ± 10 kDa, and sodium dodecyl sulfate (SDS)-PAGE results indicated that enzyme consisted of four apparently identical subunits, with a molecular weight of 72.5 ± 2.5 kDa. Kinetic characterization studies showed that optimum pH and temperature, Km and Vmax values of the purified catalase which were stable in basic region and at comparatively high temperatures were 7.5, 30°C, 289.86 mM, and 250,000 U/mg, respectively. The activity of purified catalase from P. chrysosporium was significantly inhibited by dithiothreitol (DTT), 2-mercaptoethanol, iodoacetamide, EDTA, and sodium dodecyl sulfate (SDS). It was found that while antibiotics had no inhibitory effects, 45 ppm benomyl, 144 ppm captan, and 47.5 ppm chlorothalonil caused 14.52, 10.82, and 38.86% inhibition of purified catalase, respectively. The inhibition types of these three fungicides were found to be non-competitive inhibition with the Ki values of 1.158, 0.638, and 0.145 mM and IC50 values of 0.573, 0.158, 0.010 mM, respectively. The results of in vivo experiments also showed that benomyl, captan and chlorothalonil caused 15.25, 1.96, and 36.70% activity decreases after 24-h treatments compared to that of the control.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kavakçıoğlu</LastName><ForeName>Berna</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Faculty of Science, Department of Chemistry, University of Dokuz Eylul , Izmir , Turkey.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tarhan</LastName><ForeName>Leman</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>10</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Artif Cells Nanomed Biotechnol</MedlineTA><NlmUniqueID>101594777</NlmUniqueID><ISSNLinking>2169-1401</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005659">Fungicides, Industrial</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.6</RegistryNumber><NameOfSubstance UI="D002374">Catalase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002374" MajorTopicYN="N">Catalase</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046508" MajorTopicYN="N">Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005659" MajorTopicYN="N">Fungicides, Industrial</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phanerochaete chrysosporium</Keyword><Keyword MajorTopicYN="N">catalase</Keyword><Keyword MajorTopicYN="N">fungicide</Keyword><Keyword MajorTopicYN="N">inhibition</Keyword><Keyword MajorTopicYN="N">production</Keyword><Keyword MajorTopicYN="N">purification</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24079700</ArticleId><ArticleId IdType="doi">10.3109/21691401.2013.821412</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Turquie</li></country></list><tree><noCountry><name sortKey="Tarhan, Leman" sort="Tarhan, Leman" uniqKey="Tarhan L" first="Leman" last="Tarhan">Leman Tarhan</name></noCountry><country name="Turquie"><noRegion><name sortKey="Kavakc O Lu, Berna" sort="Kavakc O Lu, Berna" uniqKey="Kavakc O Lu B" first="Berna" last="Kavakç O Lu">Berna Kavakç O Lu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000313 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000313 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24079700
|texte= In vitro and in vivo inhibitory effects of some fungicides on catalase produced and purified from white-rot fungus Phanerochaete chrysosporium.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24079700" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |